Rhodium in PDB 4gjs: Streptavidin-K121H

The structure of Streptavidin-K121H, PDB code: 4gjs was solved by T.Heinisch, T.Schirmer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.32 / 1.85
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	81.652, 81.331, 47.002, 90.00, 104.83, 90.00
R / Rfree (%)	20.3 / 25

The structure of Streptavidin-K121H also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Rhodium atom in the Streptavidin-K121H (pdb code 4gjs). This binding sites where shown within 5.0 Angstroms radius around Rhodium atom.
In total 4 binding sites of Rhodium where determined in the Streptavidin-K121H, PDB code: 4gjs:
Jump to Rhodium binding site number: 1; 2; 3; 4;

Rhodium binding site 1 out of 4 in the Streptavidin-K121H


Mono view


Stereo pair view

A full contact list of Rhodium with other atoms in the Rh binding site number 1 of Streptavidin-K121H within 5.0Å range: probe atom residue distance (Å) B Occ A:Rh400 b:64.5 occ:1.00 RH A:0OD400 0.0 64.5 1.0 C14 A:0OD400 2.1 48.5 1.0 C17 A:0OD400 2.1 54.0 1.0 C15 A:0OD400 2.1 55.7 1.0 C13 A:0OD400 2.1 45.0 1.0 C16 A:0OD400 2.1 44.3 1.0 CL1 A:0OD400 2.4 68.0 1.0 C18 A:0OD400 3.2 46.4 1.0 C21 A:0OD400 3.2 38.5 1.0 C19 A:0OD400 3.2 48.7 1.0 C12 A:0OD400 3.2 51.7 1.0 C20 A:0OD400 3.2 42.0 1.0 C11 A:0OD400 4.4 41.2 1.0

Rhodium binding site 2 out of 4 in the Streptavidin-K121H


Mono view


Stereo pair view

A full contact list of Rhodium with other atoms in the Rh binding site number 2 of Streptavidin-K121H within 5.0Å range: probe atom residue distance (Å) B Occ A:Rh401 b:44.6 occ:0.70 CE1 A:HIS127 2.8 14.5 1.0 O B:HOH574 2.8 18.2 1.0 RH1 B:RH401 3.1 42.5 0.7 ND1 A:HIS127 3.3 24.9 1.0 O A:HOH524 3.8 19.0 1.0 NE2 A:HIS127 3.9 30.3 1.0 ND1 B:HIS127 4.1 23.7 1.0 OG1 B:THR129 4.3 34.7 1.0 CE1 B:HIS127 4.3 14.6 1.0 O B:HOH549 4.5 27.1 1.0 CG A:HIS127 4.6 13.7 1.0 CD2 A:HIS127 4.9 15.0 1.0

Rhodium binding site 3 out of 4 in the Streptavidin-K121H


Mono view


Stereo pair view

A full contact list of Rhodium with other atoms in the Rh binding site number 3 of Streptavidin-K121H within 5.0Å range: probe atom residue distance (Å) B Occ B:Rh400 b:58.2 occ:1.00 RH B:0OD400 0.0 58.2 1.0 C14 B:0OD400 2.1 56.8 1.0 C17 B:0OD400 2.1 65.0 1.0 C15 B:0OD400 2.1 58.7 1.0 C13 B:0OD400 2.1 55.6 1.0 C16 B:0OD400 2.1 53.9 1.0 CL1 B:0OD400 2.4 73.1 1.0 C18 B:0OD400 3.2 48.8 1.0 C21 B:0OD400 3.2 54.7 1.0 C19 B:0OD400 3.2 49.1 1.0 C12 B:0OD400 3.2 44.5 1.0 C20 B:0OD400 3.2 36.8 1.0 C11 B:0OD400 4.4 45.7 1.0

Rhodium binding site 4 out of 4 in the Streptavidin-K121H


Mono view


Stereo pair view

A full contact list of Rhodium with other atoms in the Rh binding site number 4 of Streptavidin-K121H within 5.0Å range: probe atom residue distance (Å) B Occ B:Rh401 b:42.5 occ:0.70 CE1 B:HIS127 2.7 14.6 1.0 O A:HOH524 2.7 19.0 1.0 RH1 A:RH401 3.1 44.6 0.7 ND1 B:HIS127 3.3 23.7 1.0 O B:HOH574 3.7 18.2 1.0 NE2 B:HIS127 3.8 29.9 1.0 ND1 A:HIS127 4.2 24.9 1.0 OG1 A:THR129 4.2 35.5 1.0 O A:HOH555 4.3 27.1 1.0 CE1 A:HIS127 4.5 14.5 1.0 CG B:HIS127 4.6 16.2 1.0 CD2 B:HIS127 4.8 16.1 1.0 O B:HOH610 4.9 43.4 1.0

J.M.Zimbron, T.Heinisch, M.Schmid, D.Hamels, E.S.Nogueira, T.Schirmer, T.R.Ward. A Dual Anchoring Strategy For the Localization and Activation of Artificial Metalloenzymes Based on the Biotin-Streptavidin Technology. J.Am.Chem.Soc. V. 135 5384 2013.
ISSN: ISSN 0002-7863
PubMed: 23496309
DOI: 10.1021/JA309974S